ANTIBACTERIAL ACTIVITY OF Gynura procumbens LEAF EXTRACT AGAINST Pseudomonas aeruginosa IN VITRO
Abstract
Intensification of fish farming has caused various impacts, for example diseases in fish. One of the diseases in fish is a bacterial disease. The use of natural compounds as an antimicrobial agents, which is relatively safe and effective, is a strategy to treat the disease. The aim of this study was to evaluate the antibacterial activity of Gynura procumbens leaf extract against Pseudomonas aeruginosa. Antibacterial activity test was done by using paper disc diffusion. The variations on the test paper disc method used were based on the concentration difference of Gynura procumbens leaf extract such as: A (250 ppm), B (500 ppm), C (750 ppm), D (1.000 ppm) dan E (1.250 ppm). The results showed Gynura procumbens leaf extract produced antibacterial compounds which inhibited the growth of the bacteria. Effective antibacterial activity was detected in concentration of 1.250 ppm with inhibition zone diameter of 11,01 ± 0,59 mm. It can be concluded that Gynura procumbens leaf extract may contribute in controlling the spread of bacterial diseases in fish farming, particularly caused by Pseudomonas aeruginosa.
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